Saturday, February 4, 2012

stephi fundamentalism: Sports, Fitness and Recreation: Dunlop ...

There are many makes which generate diverse sporting products. A number of have been in the business for a long time. In this post, we will probably describe what a Dunlop squash is dependant on as well as exactly how you can certainly gain benefit from the make use of it. The model makes use of engineering that?s very advanced so that you can build and also production their own squash racquets that will really stay the check of time frame. They make use of an Advanced Control System (ACS) technologies which often will work to

Sports Fitness and Recreation

Source: http://sportsfitnessrecreation.blogspot.com/2012/01/dunlop-squash-supplies-maded-by-product.html

matt ryan matt ryan real housewives of new york mildred pierce cam newton emmy awards nick collins

Source: http://resortsspanishtranslation.ato-wsu.com/75/sports-fitness-and-recreation-dunlop-squash-supplies-maded-by/

yom kippur diamondbacks wolf creek wolf creek arizona diamondbacks arizona diamondbacks alex rodriguez

Source: http://stephi-fundamentalism.blogspot.com/2012/02/sports-fitness-and-recreation-dunlop.html

apple announcement yu darvish pipa keystone xl sopa bill sopa and pipa piracy

US diplomat urges Vietnam to improve human rights (AP)

HANOI, Vietnam ? A senior U.S. diplomat says Vietnam should improve its human rights record in order to make progress in its relationship with the United States.

Assistant Secretary of State Kurt Campbell says Vietnam's stance on human rights is "antithetical" to otherwise warming economic and military ties between the two countries.

Campbell, the top U.S. diplomat for East Asia, spoke to reporters Thursday in Hanoi.

He declined to mention specific cases, but said Vietnam still holds political prisoners.

Rights groups regularly accuse Vietnam of human rights violations.

In January, the U.S. Embassy urged Vietnam to release Bui Thi Minh Hang, an activist who in November was sent without trial to a drug rehabilitation camp for two years.

Source: http://us.rd.yahoo.com/dailynews/rss/asia/*http%3A//news.yahoo.com/s/ap/20120202/ap_on_re_as/as_vietnam_human_rights

x factor judges raiders news raiders news ice cream sandwich android ice cream sandwich android harry belafonte harry belafonte

Friday, February 3, 2012

'Yellow biotechnology': Using plants to silence insect genes in a high-throughput manner

ScienceDaily (Feb. 2, 2012) ? 'Yellow biotechnology' refers to biotechnology with insects -- analogous to the green (plants) and red (animals) biotechnology. Active ingredients or genes in insects are characterized and used for research or application in agriculture and medicine. Scientists at the Max Planck Institute for Chemical Ecology in Jena, Germany, are now using a procedure which brings forward ecological research on insects: They study gene functions in moth larvae by manipulating genes using the RNA interference technology (RNAi). RNAi is induced by feeding larvae with plants that have been treated with viral vectors. This method -- called "plant virus based dsRNA producing system" (VDPS) -- increases sample throughput compared to the use of genetically transformed plants.?

Natural toxins against herbivores

More than 200,000 insects species are herbivores. They depend on plants for food and have adapted their metabolism accordingly in the course of evolution to render plant defenses, such as the toxins plants produce to fend off herbivores, ineffective. The operating instructions of these detoxification processes are coded in different genes. Insects have evolved an enormous diversity of adaptation mechanisms; they colonize most habitats on this planet -- which makes them interesting research objects in ecological studies. Which insect species attack which plants species? Which toxins or signaling substances are involved? Has the insect species adapted to one specific plant species or is it a food generalist? Interesting for agriculture: Which genes allow particular pest insects, such as the pollen beetle Meligethes aeneus or the Western corn rootworm Diabrotica virgifera virgifera, to be so destructive to crop plants? Knowing these detoxification genes and switching them off with the consequence that plant toxins are no longer effective, is currently a research subject in plant breeding. First success stories have already been reported -- thanks to the use of RNAi technology.

Scientists at the Max Planck Institute for Chemical Ecology examined a well-known plant toxin: nicotine. Plants of the species Nicotiana attenuata (coyote tobacco) produce nicotine as a defensive substance against herbivores. However, it does not have any toxic effects on their worst enemy: larvae of the tobacco hornworm Manduca sexta. The insect is resistant against this alkaloid; genes that encode nicotine-catabolizing enzymes may be responsible for its resistance. These so called CYP genes are involved in the formation of cytochrome P450 enzymes; the expression of some of these genes is increased as soon as the insect larvae are exposed to nicotine in their food. Ian Baldwin and his team identified the DNA sequences of CYP genes in Manduca sexta and were able to switch off these genes using RNAi technology, but expressed in the plant.

Using plants to silence insect genes

RNA interference (RNAi) is triggered by the production of double-stranded RNA (dsRNA) comprising about 300 base pairs in the cells of tobacco plants. If larvae feed on these plant, the RNA is released in the insect gut. In the experiments, the dsRNA harbored the sequence of the insect gene,CYP6B46, a special cytochrome P450 oxidoreductase specific for Manduca sexta larvae. In a next step, the dsRNA was enzymatically broken down into smaller RNA segments; a special enzyme complex called RISC (RNA-induced silencing complex), which carries several of these RNA segments, specifically binds to the messenger RNA (mRNA) of the CYP6B46 gene and disassembles the mRNA in such a way that the cytochrome P450 enzyme cannot be produced anymore. "We were impressed by the high specificity of these RNAi experiments. The analysis of mRNA transcripts of closely related CYP6 genes revealed that only the CYP6B46 gene was silenced. This means that there was no collateral damage from the procedure: the gene silencing worked on only one targeted gene," says Ian Baldwin.

The use of additional CYP RNAi probes revealed further interesting results: Young caterpillars which had ingested dsRNA of the CYP4M3 gene gained significantly less weight within 14 days in comparison to larvae reared on control plants -- very likely a consequence of the nicotine and its toxic effect which had been restored by switching off the CYP gene. The RNAi experiments had been conducted using plant viral vectors. Unlike genetically transformed tobacco plants in which CYP dsRNA is produced constitutively, the virus vector-based technique provides dsRNA transiently produced in wildtype tobacco plants. Both methods worked well but the "plant virus-based dsRNA producing system" (VDPS) allows for a throughput of RNAi samples that is four times faster. Many unknown functions of different insect genes involved in the adaptation of insects to their environment can now be analyzed using the VDPS technique.

However, it is still unclear how the individual steps in the RNAi mechanism -- from producing dsRNA in the plant cell via their uptake in the insect gut to the silencing of the detoxification genes -- are accomplished to induce a maximum effect. One experiment provided some interesting information: If the enzymatic step which dices dsRNA into small fragments is inhibited in the experimental plants, the amount of transcripts of the detoxification gene was reduced even further. Therefore the plant mediated RNAi procedure may be more effective, if the caterpillars ingest complete dsRNA instead of smaller diced RNA segments.

Recommend this story on Facebook, Twitter,
and Google +1:

Other bookmarking and sharing tools:


Story Source:

The above story is reprinted from materials provided by Max Planck Institute for Chemical Ecology.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Pavan Kumar, Sagar Subhash Pandit, Ian T. Baldwin. Tobacco Rattle Virus Vector: A Rapid and Transient Means of Silencing Manduca sexta Genes by Plant Mediated RNA Interference. PLoS ONE, 2012; 7 (2): e31347 DOI: 10.1371/journal.pone.0031347

Note: If no author is given, the source is cited instead.

Disclaimer: Views expressed in this article do not necessarily reflect those of ScienceDaily or its staff.

Source: http://www.sciencedaily.com/releases/2012/02/120202151248.htm

stanford vs oklahoma state caucus vesta williams occupy rose parade stanford stanford oklahoma state university

Tunisia's Chedli walks out on team at African Cup

By GERALD IMRAY

AP Sports Writer

Associated Press Sports

updated 12:09 p.m. ET Feb. 1, 2012

FRANCEVILLE, Gabon (AP) -Tunisia midfielder Adel Chedli has walked out on his squad at the African Cup of Nations after he was overlooked for selection.

Team spokesman Ward Zouhair tells The Associated Press that Chedli "wasn't happy" at not playing and left the team's base in Franceville on Wednesday morning, four days head of the country's quarterfinal.

Zouhair says the 35-year-old veteran, who won the African Cup with Tunisia in 2004, is in Libreville waiting for a flight home and won't play any further part at the tournament.

Zouhair adds there was no problem with Chedli during Tuesday's 1-0 loss to Gabon at Stade de Franceville despite reports he was sent to the stands by coach Sami Trabelsi after becoming angry that he wasn't in the team.

? 2012 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.


advertisement

More newsAFP - Getty Images
Terry to stand trial for racism

??England captain John Terry will stand trial after the European Championship over allegations he racially abused an opponent during a Premier League match ? possibly clearing the way for him to play in the tournament.

Source: http://nbcsports.msnbc.com/id/46222203/ns/sports-soccer/

real housewives of new york mildred pierce cam newton emmy awards nick collins cape coral fl emmys

Thursday, February 2, 2012

InsideView Announces General Availability of People Insights(TM ...

By Marketwire .

Article Rating:

February 1, 2012 01:16 PM EST

Reads:

188

SAN FRANCISCO, CA -- (Marketwire) -- 02/01/12 -- InsideView, the only social selling provider to reveal the person behind the contact, today announced general availability of People Insights?, a unique sales intelligence solution that combines an enterprise-wide social graph of connections to prospects that leverage a organization's network of customers, employees and executives, a complete social profile of individuals, and sales alerts based on meaningful professional events that can signal sales opportunities. InsideView People Insights arms sales professionals with actionable intelligence from social sources, media outlets, CRM records and relevant personal connections that help to build profitable, trusted relationships with prospects and customers.

Using the enterprise-wide social graph capabilities in InsideView People Insights, Network Hardware Resale (NHR), a California-based provider of networking solutions has changed the way it assigns leads. Sales leadership uses the solution to identify connections between staff members and prospects. Sales professionals are then assigned leads based on professional connections. Unlike territory-based lead assignments, connection-based lead assignments empower sales executives with warm connections or introductions from trusted colleagues. These connections are augmented with sales intelligence from InsideView. The result is stronger sales relationships, shorter sales cycles, and more sales success.

Michael Lodato, senior vice president of sales and marketing for NHR commented: "The days of cold calling or blast emails are over for us. Social connections are driving a fundamental change in the way we approach prospects. Successful sales engagements are based on trusted relationships. With InsideView, my sales team has access to the insight they need about each person in the CRM system. They know how and when to make contact and they are often able to leverage a warm introduction from a colleague on the first touch. We are already seeing results in our bottom line."

Industry research shows that 90 percent of executives never respond to cold calls or unsolicited emails, yet 84 percent will engage with a sales person when they are connected through a friend, colleague, customer, or industry peer.* To turn cold calls into warm introductions, InsideView People Insights includes the following capabilities:

  • Enterprise-wide Connection Sharing -- extends connections beyond social networking sites to automatically leverage the network of coworkers, executives and board members, previous employers, and customers. This allows all sales team members, even new hires, to instantly leverage their company's extended network of professional relationships.
  • New social content -- content from social sources including Facebook, Twitter, RSS feeds, blogs and other sources creates deeper insights for building profitable sales relationships.
  • InsideView People Alerts -- tracks key people across companies and industries so that the sales team is the first to be notified about job changes, news mentions, social media chatter, and other key developments. These alerts are integrated with email and CRM applications so that sales professionals always know the right time to make contact.

"Social media is changing the way successful companies do business by shifting sales professionals' focus to meaningful insights and enterprise connections that build relationships," said Umberto Milletti, CEO of InsideView. "Only InsideView People Insights looks across all relevant touch points and puts actionable insights at the fingertips of sales people. People Insights is one example of how we continue to reshape old-school sales practices and enable sales professionals to quickly build trusted relationships and win more deals."

InsideView People Insights is now available from InsideView and our certified Trusted Advisor Partners. Like all InsideView products, it is integrated into all leading CRM solutions. To learn more about InsideView People Insights, please visit our website.

About InsideView
InsideView, the leading provider of sales intelligence, increases productivity and revenue by delivering relevant business and social insights to the point of need. Our award-winning technology gathers and analyzes information from the most relevant social media, user-contributed and traditional/proprietary editorial sources to provide compelling insights about companies and contacts directly within your CRM, browser, or mobile device. More than 100,000 sales professionals, and over 1,000 market-leading companies including Adobe, AIG, BMC, CapGemini, Experian, and SuccessFactors use InsideView. For more information, visit InsideView.

*Kenan-Flagler Business School, Selling to the C-Suite, published August 2009

Add to Digg Bookmark with del.icio.us Add to Newsvine

Source: http://buyersteps.ulitzer.com/node/2150647

dallas news google tv cornel west marzieh vafamehr marzieh vafamehr lady liberty lady liberty

Westward, Ho: Romney, GOP rivals now spreading out

Republican presidential candidate, former Massachusetts Gov. Mitt Romney greets supporters at a campaign rally in Eagan, Minn., Wednesday, Feb. 1, 2012. (AP Photo/Gerald Herbert)

Republican presidential candidate, former Massachusetts Gov. Mitt Romney greets supporters at a campaign rally in Eagan, Minn., Wednesday, Feb. 1, 2012. (AP Photo/Gerald Herbert)

Republican presidential candidate, former House Speaker Newt Gingrich shakes hands during a campaign stop at the Great Basin Brewing Co. Wednesday, Feb. 1, 2012, in Reno, Nev. (AP Photo/Evan Vucci)

Republican presidential candidate Mitt Romney left, hands 6-month-old Dexter Hall of Plymouth, Minn. back to his mother, Laura, during a campaign stop at Freightmasters, Inc. in Eagan, Minn., on Wednesday, Feb. 1, 2012. Romney, who won big in Florida with a barrage of negative ads, predicted the tone of the GOP campaign was "just a precursor to what you'll see" from President Barack Obama in the general election. And he said voters paid more attention to what they heard in the campaign debates than whatever ads were flooding the airwaves. (AP Photo/The St. Paul Pioneer Press, Ben Garvin ) MINNEAPOLIS STAR TRIBUNE OUT

A Reno police officer looks over a crowd as Republican presidential candidate former House Speaker Newt Gingrich makes a campaign stop at the Great Basin Brewing Co. on Wednesday, Feb. 1, 2012, in Reno, Nev. (AP Photo/Evan Vucci)

Republican presidential candidate, former Pennsylvania Sen. Rick Santorum greets students during a campaign stop, Wednesday, Feb 1, 2012, at Colorado Christian University in Lakewood, Colo. (AP Photo/Ed Andrieski)

WASHINGTON (AP) ? The Republican presidential campaign rolled westward Wednesday, Mitt Romney riding herd after his Florida primary victory, Newt Gingrich looking for a new stake and a top party leader insisting the long trail won't necessarily hurt the GOP in the race against President Barack Obama.

Already the television ads were showing up in states that vote next, caucuses in Nevada on Saturday, in Minnesota and Colorado next Tuesday and in Maine the following weekend.

"I'm feeling like we've got a good pathway ahead," Romney declared in a television interview on the day after his Florida triumph.

He is favored in Nevada, where there are 28 Republican National Convention delegates at stake. And, alone among the contenders, appears to have the money to compete aggressively in all the other states as well.

Gingrich decamped from Florida but with prospects considerably dimmer than Romney's.

He was conceding nothing. Routed on Tuesday night, the former House speaker vowed to stay in the race until the party convention next summer. And his decision not to telephone the primary winner with congratulations drew notice.

"I guess Speaker Gingrich doesn't have our phone number," Romney said.

The Florida campaign was marked by millions of dollars in negative ads, and Gingrich's promise to remain in the race raised the prospect of a months-long struggle. But current House Speaker John Boehner said he was not concerned.,

"I would remind people that President Obama and Secretary of State Hillary Clinton had a fight that went through June of 2008. I think everybody just needs to realize that this will resolve itself," he said.

Jubilant in victory on Tuesday night, Romney was thrown onto the defensive the day after. "I'm not concerned about the very poor" because they have a social safety net, he said on CNN. Criticized quickly, he hastened to clarify.

"No, no, no, no, no, no, no," Romney told reporters on his campaign plane when asked about the comments. He referred back to his complete remarks, in which he had said he would focus on middle-income Americans rather than the very poor, who get government help, or the very rich, who don't need it. "My energy is going to be devoted to helping middle-income people," he said.

By then, though, he was drawing criticism from conservatives who worried he was showing a penchant for verbal gaffes as well as from Obama's campaign manager. "So much for 'we're all in this together,'" tweeted Jim Messina.

Gingrich piled on. " I am fed up with politicians in either party dividing Americans against each other," he said.

After making no significant campaign effort in Florida, former Sen. Rick Santorum and Texas Rep. Ron Paul looked for better days in the contests just ahead.

Santorum picked up an endorsement from former Colorado Rep. Tom Tancredo, known for his hardline position on immigration.

Eager to emerge as the leading conservative in the race, Santorum said Gingrich was nearing the end of his run. "If Newt's out of the race, all his votes come to me," he said, mirroring what the former House speaker frequently says about him.

In a speech in Woodland Park, Colo., Santorum said Romney's nomination would doom the party to defeat in the fall.

"Barack Obama, in a debate or in this election, is going to destroy Mitt Romney on the issue of health care," Santorum warned, saying that the former governor supported a requirement for individuals to purchase health care in Massachusetts that is akin to the provision in the legislation that Obama signed into federal law.

Paul, campaigning in Las Vegas, said he favors an immigration policy that doesn't rely on "barbed wire fences and guns on the border." Appearing before an audience of Hispanics, he said he opposes illegal immigration but also is against any effort to round up and deport individuals who are in the United States illegally.

After a month in which four contests produced three winners, Romney appeared to hold formidable advantages in fundraising and organization to go with a lead in national convention delegates. After winning all 50 at stake in Florida, he had 87 in AP's count. Gingrich had 26 delegates, Rick Santorum had 14 and Ron Paul had four. It takes 1,144 to win the nomination.

Reports filed with the Federal Election Commission show Romney's campaign had $20 million on hand as of Jan. 1 and Restore Our Future, an outside group that supports him, had $23.6 million.

The total of $43.6 million dwarfs figures reported by the other contenders and the groups that back them, although the onset of the 2012 caucuses and primaries was certain to have produced changes.

Figures from Florida show Romney and Restore Our Future spent more than $15 million on television ads combined, many of which attacked Gingrich. The former speaker and an organization that backs him spent less than $4 million.

Already, Romney and Restore our Future were airing ads in Nevada, as was Paul.

Paul also was on the air in Minnesota, along with the Red White and Blue Fund, which support Santorum.

___

Associated Press writers Philip Elliott and Beth Fouhy in Colorado, Kasie Hunt in Minnesota, Laurie Kellman in Washington and Shannon McCaffrey in Nevada contributed to this story.

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2012-02-01-US-GOP-Campaign/id-09e26ae63e9e44efb14a376116e9aa44

whats your number whats your number melissa gorga melissa gorga pueblo co pueblo co pineapple express

Wednesday, February 1, 2012

Megaupload users' data to be kept another two weeks, EFF to help folks retrieve it

Megaupload's digital doors may have been closed due to the presence of pirated materials, but there's still the matter of all that legal content residing on its servers. Naturally, folks want their files back, but now that the government's gotten what it needs, the hosting companies no longer need to keep the data around because Megaupload's no longer paying them to do so. Carpathia Hosting and Cogent Communications, however, have decided to preserve the data for another two weeks while a deal is brokered with the DOJ for its release. In the meantime, the Electronic Frontier Foundation (EFF) has teamed up with Carpathia to create a website that puts folks in touch with EFF attorneys so users can try to retrieve their data. No word as to what legal wrangling the EFF can do to make it happen, but those affected can get the wheels of justice started at the source below.

Megaupload users' data to be kept another two weeks, EFF to help folks retrieve it originally appeared on Engadget on Tue, 31 Jan 2012 21:19:00 EDT. Please see our terms for use of feeds.

Permalink The Inquirer, CNET  |  sourceMegaRetrieval.com  | Email this | Comments

Source: http://www.engadget.com/2012/01/31/megaupload-users-data-to-be-kept-another-two-weeks-eff-to-help/

cma awards christmas tree tax cmas cmas world series of poker joe walsh zsa zsa gabor